Isolation of L-Asparaginase Producing Endophytic Bacteria from Plants Recommended for Cancer Therapy
نویسنده
چکیده
Endophytic microorganisms are recognized as potential source of novel chemical molecules that might be useful in the treatment of infectious diseases. This study was conducted with the aim to isolate and identify endophytic bacteria from medicinal plants recommended for cancer therapy with a potential to produce L-asparaginase. Five different plants were selected to enhance diversity as Catharanthus roseus, Oscimum sanctum, Alovera, Withania somnifera and Morrayo konengi. Isolation of endophytic bacteria was done on Tryptic soy agar media and characterized on the basis of morphological and biochemical characteristics. A total 45 bacterial endophytes were obtained out of which 25 showed varied levels of L-asparaginase activity.
منابع مشابه
Isolation and identification of L-asparaginase producing actinomycetes from Persian Gulf
Introduction: L-asparaginase is an anti-neoblastic agent used in the chemotherapy of lymphoblastic leukemia. This enzyme is widely distributed among microorganisms, plants and animals, but microorganisms have proved to be a better alternative for L-asparaginase, thus facilitating its large-scale production. Actinomycetes are filamentous, Gram-positive bacteria widely distributed in the marine e...
متن کاملL-Asparaginase-producing Rouxiella Species Isolation, Antileukemia Activity Evaluation, and Enzyme Production Optimization
Background: L-Asparaginase (L-Asp) is used as an efficient anti-cancer drug, especially for acute lymphoblastic leukemia (ALL). Currently, two bacterial asparaginase isoenzymes are used for cancer treatment. Therefore, this research focused on isolating native bacteria with the ability to produce L-Asp. Materials and methods: L-Asp producing bacteria were isolated from soil samples on 9K medi...
متن کاملEndophytic l-asparaginase-producing fungi from plants associated with anticancer properties
Endophytes are novel sources of natural bioactive compounds. This study seeks endophytes that produce the anticancer enzyme l-asparaginase, to harness their potential for mass production. Four plants with anticancer properties; Cymbopogon citratus, Murraya koenigii, Oldenlandia diffusa and Pereskia bleo, were selected as host plants. l-Asparaginase-producing endophytes were detected by the form...
متن کاملL-asparaginase production in the pseudomonas pseudoalcaligenes strain JHS-71 isolated from Jooshan Hot-spring
L-asparaginase has lots of medical and industrial applications. Ever since L-asparaginase anti-tumor activity was first demonstrated, its production using microbial systems has attracted considerable attention owing to their cost-effective and eco-friendly nature. The aim of this study is to obtain L-asparaginase producing bacteria and determining the enzyme activity. Samples were picked up fro...
متن کاملA Review on Isolation and Identification of Endophytic Actinobacteria, Their Chemical Structure, Bioactive Compounds, and Potential Medical-Pharmaceutical Applications
Over recent years, nosocomial infections, and the morbidity and mortality associated with pathogenic bacteria have dramatically increased due to antibiotic resistance and imposed significant burdens on the global health system. Critical shortage of effective therapeutics against multidrug-resistant bacteria highlights the need for development of novel antibiotics. Actinobacteria are well-known...
متن کامل